Bioleaching and beneficiation of agbaja iron ore using Providencia vermicola KUBT-1 under varying process conditions

Authors

  • Chibuzor Nwadibe Eze University of Nigeria, Nsukka
  • Maria Nneka Ugwoji University of Nigeria, Nsukka
  • Obianuju Nnabuife University of Nigeria, Nsukka
  • Simeon Chukwuemeka Enemuor University of Nigeria, Nsukka
  • Vanessa Okechukwu University of Nigeria, Nsukka
  • Christian Kelechi Ezeh University of Nigeria, Nsukka

DOI:

https://doi.org/10.17159/

Abstract

In this study, the efficacy of Providencia vermicola KUBT-1 in the leaching and beneficiation of Agbaja iron ore was evaluated under varying conditions of temperature,  ore particle size  and pH.  Agbaja iron ore is a Nigerian low grade ore containing about 43-52% iron and high amount of gangue materials including phosphorus (1.4-2.0%) and sulphur (0.12%). The study was organized in triplicates in shake flasks with a total of 48 samples.  One millilitre of washed cells of Providencia vermicola KUBT-1 suspended in normal saline at 0.5 McFarland standard (108 cells ml-1) was introduced into each flask containing 50g of sterilized ore in 100ml of bioleaching medium and the samples incubated at room temperature (250C – 300C) for eight weeks.  The levels of upgraded iron and mobilized sulphur and phosphorus were determined by Atomic Absorption and Ultraviolet Spectrophotometric analyses respectively.  Results obtained showed that a particle size of 0.8mm was optimal for iron upgrading and sulphur mobilization while 1.0mm ore size was optimal for phosphorus mobilization. Up to 89.60% of the iron was upgraded while phosphorus and sulphur were mobilized leaving residual values of 0.223% and 0.02% respectively in the ore. The organism produced best beneficiation results at a pH of 2.5 and a temperature of 30oC.  Correlation analysis with the Pearson’s correlation model showed a positive correlation between iron upgrading and sulphur-phosphorus removal (r =1.000, and r =0.967) respectively.  These results present Providencia vermicola KUBT-1 as a promising candidate for large scale leaching and beneficiation of Agbaja iron ore.

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Published

2026-01-19

Issue

Section

Environmental, Social and Governance