Bioleaching and beneficiation of agbaja iron ore using Providencia vermicola KUBT-1 under varying process conditions
DOI:
https://doi.org/10.17159/Abstract
In this study, the efficacy of Providencia vermicola KUBT-1 in the leaching and beneficiation of Agbaja iron ore was evaluated under varying conditions of temperature, ore particle size and pH. Agbaja iron ore is a Nigerian low grade ore containing about 43-52% iron and high amount of gangue materials including phosphorus (1.4-2.0%) and sulphur (0.12%). The study was organized in triplicates in shake flasks with a total of 48 samples. One millilitre of washed cells of Providencia vermicola KUBT-1 suspended in normal saline at 0.5 McFarland standard (108 cells ml-1) was introduced into each flask containing 50g of sterilized ore in 100ml of bioleaching medium and the samples incubated at room temperature (250C – 300C) for eight weeks. The levels of upgraded iron and mobilized sulphur and phosphorus were determined by Atomic Absorption and Ultraviolet Spectrophotometric analyses respectively. Results obtained showed that a particle size of 0.8mm was optimal for iron upgrading and sulphur mobilization while 1.0mm ore size was optimal for phosphorus mobilization. Up to 89.60% of the iron was upgraded while phosphorus and sulphur were mobilized leaving residual values of 0.223% and 0.02% respectively in the ore. The organism produced best beneficiation results at a pH of 2.5 and a temperature of 30oC. Correlation analysis with the Pearson’s correlation model showed a positive correlation between iron upgrading and sulphur-phosphorus removal (r =1.000, and r =0.967) respectively. These results present Providencia vermicola KUBT-1 as a promising candidate for large scale leaching and beneficiation of Agbaja iron ore.
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Copyright (c) 2026 Chibuzor Nwadibe Eze, Maria Nneka Ugwoji, Obianuju Nnabuife, Simeon Chukwuemeka Enemuor, Vanessa Okechukwu, Christian Kelechi Ezeh

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